Process Development
Light scattering measurements help process development in several ways:
Purification end point
Since multi-angle light scattering determines molar masses independently of retention time, RT-MALS is ideal for real-time characterization of purification products and determination of end points. For more detailed product characterization during process development, SEC-MALS may be implemented at-line with UHPLC for rapid separation and analysis.
Quaternary structure
Complex products such as virus-like particles or peptide micelles must be produced with specific masses, shapes and sizes, as well as minimum quantities of residual unassembled material. SEC-MALS or FFF-MALS with on-line DLS validates such processes to quantify unassembled sub-units and ensure that the final product conforms to the required structure.