Payload quantification

ASTRA's LNP Analysis module quantitates the nucleic acid weight fraction using online MALS, UV260 and dRI detectors coupled to SEC or FFF. The same data simultaneously quantify many other quality attributes, such as size distribution, particle concentration, morphology, encapsulation efficiency, mRNA concentration and lipid concentration. The method was validated against conventional offline measurements in “Enabling online determination of the size-dependent RNA content of lipid nanoparticle-based RNA formulations.”