mRNA/DNA

As described in application note AN1616: SEC-MALS methods for characterizing mRNA, key biophysical attributes of mRNA are quantified by SEC-MALS plus DLS. This analysis determines molecular weight, percentage of aggregates, radius of gyration Rg and hydrodynamic radius Rh. The relationship between Mw and Rg identifies single- or double-stranded RNA, while the ratio Rg:Rh indicates molecular conformation. This method can be readily adopted to further study how these attributes are affected by mRNA preparation, formulation and storage conditions.