Quality and Quantity of AAV, genetic payload, and associated proteins

Size-exclusion chromatography (SEC) combined with multi-angle light scattering (MALS) is ideal for separation, characterization, and quantification of a wide range of gene therapy vectors, their genetic payload, and other proteins associated with gene therapy development. This method uses a DAWN™ MALS detector combined with an Optilab™ differential refractive index (dRI) detector and HPLC UV module to measure absolute molar mass, size, and concentration of proteins, nucleic acids, and biological nanoparticles.