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Wyatt · Proteins

SAXS and SANS

Light scattering in the service of SAXS and SANS

Beam time is expensive and hard to come by. In order to make the most of your SAXS and SANS measurements you'll want to assure the quality of your biomolecules before they reach the chamber.

DLS is a quick and easy method to check for gross aggregation in your samples. You can use at little as 1 μL in the DynaPro NanoStar's microCuvette, and even recover it if necessary! Just 4 μL are necessary with our proprietary disposable cuvettes.

For further characterization of oligomers as well as comparison with the x-ray or neutron scattering results, SEC-MALS combining your favorite SEC apparatus with a miniDAWN MALS detector and Optilab RI detector is highly desirable. See Bazin et al., "Structure and primase-mediated activation of a bacterial dodecameric replicative helicase" Nucleic Acids Res., 2015, 1