RapiZyme RNases
Tunable RNA digestion for streamlined LC-MS sequencing
CRISPR sgRNA and mRNA require vigorous characterization to confirm their identity, sequences, and purity. These RNA molecules are prime candidates for LC-MS analysis due to their complex functionality, modifications, and variants present. Because of their size, digestion with an endonuclease such as an RNase is often performed prior to injection. The resulting digestion products can be readily detected using ion pairing reversed phase (IPRP) or hydrophilic chromatography (HILIC) coupled with mass spectrometry (MS).
Waters RapiZyme MC1 and RapiZyme Cusativin offer controlled, reproducible RNA cleavage at a range of dinucleotide sites. RapiZyme RNases produce a variety of unique RNA fragments with complementary overlap. For LC-MS users, this gives tunable digestions that improve confidence in peak identifications with a result to increase sequence coverage and molecular insights. Each tube includes 10,000 units of RapiZyme RNase, which is enough enzyme reagent to complete up to 100 digestion reactions of 10 µg RNA samples.
Achieve maximum sequence coverage for mRNA and sgRNA
Work with RNases specifically designed for LC-MS analysis
Eliminate the need for denaturants and RNases inhibitors when performing digestion
Achieve cleaner signal and sensitivity with cyclic phosphate products
Accelerate the development and analysis of RNA therapeutics