Gene Therapy
Analytical techniques based on light scattering are essential for quantifying quality attributes and other characteristics of gene therapy nanoparticles. Dynamic light scattering (DLS) and multi-angle light scattering (MALS) coupled to separation technologies (SEC-MALS, FFF-MALS) reveal molar mass and size, aggregation, physical titer, empty:full ratio and stability.
Continue reading to learn about Wyatt solutions to industry and regulatory needs for AAVs and other DNA/RNA delivery modalities. Light scattering techniques are simple to operate and enhance productivity while directly accessing the core biophysical properties that define these CQAs.
High-throughput size, stability, and quantity
Dynamic light scattering (DLS) provides rapid, low-volume, non-destructive measurements of a wide range of delivery vehicles, including adeno-associated virus…
Explore
Quality and Quantity of AAV, genetic payload, and associated proteins
Size-exclusion chromatography (SEC) combined with multi-angle light scattering (MALS) is ideal for separation, characterization, and quantification of a wide…
Explore
Aggregates, large viruses, and mRNA
While SEC-MALS measures quality attributes across a wide range of sizes, the technique is limited with respect to larger particles and aggregates. This is…
ExploreAAV Method Implementation & Training
Wyatt has developed a personalized AAV training program that saves significant time and effort in learning, method development and validation.
Explore