Proteins
Proteins and related biomacromolecules are complex entities that exhibit fascinating behavior when interacting with other biomolecules. Light scattering provides a simple and effective means for characterizing the essential biophysical properties of proteins: molar mass, size, charge, interactions, conjugation and conformation.
The DAWN™ and miniDAWN™ multi-angle light scattering detectors interfaces with any SEC system for determination of absolute molar mass via SEC-MALS and ASTRA™ software.
Protein sizes are determined by adding a WyattQELS™ DLS module into the DAWN or miniDAWN.
The DAWN also combines with the Calypso™ composition-gradient system for the study of biomolecular interactions via CG-MALS and CALYPSO™ software.
Rapid assessments of purity, aggregation and oligomerization may be made through size distributions via dynamic light scattering using a microwell-plate-based DynaPro™ Plate Reader or a DynaPro NanoStar™ microcuvette-based instrument and the DYNAMICS™ software.
Molecular charge of fragile biomolecules is determined by combining simultaneous electrophoretic mobility and size measurements in the DynaPro ZetaStar™ using DYNAMICS.
Identification
Molar mass is the key to identifying proteins, their oligomers or complexes, yet all too many protein researchers rely on simplistic, invalid analysis of…
ExploreConjugated & Membrane Proteins
Membrane proteins solubilized with detergent are particularly difficult to analyze by traditional techniques or even by mass spectroscopy because of the…
ExplorePurification & Aggregates
Scientists carrying out detailed mechanistic studies of proteins and their biological function can't afford to work with poor quality material. Light…
ExploreNative Oligomeric State
Just what is a 'native oligomeric state'? The answer may surprise you since 'native' is a relative term. Most biological oligomers consist of proteins in a…
ExploreBiomolecular Interactions
The binding of proteins into reversible complexes underlies much of biology as we know it, whether for purposes of signaling, formation of cellular structures,…
ExploreSAXS and SANS
Light scattering in the service of SAXS and SANS
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