DLS: High-throughput for formulation screening and process characterization

DLS provides rapid, low-volume, non-destructive AAV quantification to determine size, size distribution and physical titer. Typical experiment times are 30 seconds per sample.

AAV characterization by DLS is essential for:

Monitoring titer of fractions during AAV production, especially in downstream processes

Screening buffers and serotypes for colloidal stability and propensity to aggregate

Assessing thermal stability by measuring the aggregation onset temperature, melting temperature, or the temperature dependence of aggregation kinetics

Identifying SEC mobile phase or formulation buffers that promote well-assembled product

The DynaPro™ Plate Reader is the premier tool for high-throughput DLS: it evaluates product quality, quantity and stability for up to over a thousand samples at a time. Since the DynaPro Plate Reader measures directly in standard microwell plates, consumable and labor costs are minimal, and common automation tools such as robotic pipettors may be incorporated in the workflow.