AAVs
Adeno-associated viruses (AAVs) have emerged as one of the main delivery vehicles for gene therapies. With their rapid growth in development and production, it is essential to implement simple, fast AAV quantification methods for accurately determining the critical quality attributes (CQA) of AAV serotypes, as well as providing extended characterization. Analytical methods based on dynamic light scattering (DLS) and multi-angle light scattering (MALS) are advantageous for quantifying CQAs and other characteristics of AAV vectors: they require no special reagents, extensive sample preparation or calibration curves, are highly reproducible, and results are obtained in just seconds to minutes.
Click on the buttons to explore how light scattering is used to measure different properties of viral vectors or continue reading to learn about Wyatt solutions to industry and regulatory needs for AAVs.
AAV analytical methods based on light scattering
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DLS: High-throughput for formulation screening and process characterization
DLS provides rapid, low-volume, non-destructive AAV quantification to determine size, size distribution and physical titer. Typical experiment times are 30…
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FFF-MALS: Extended aggregate characterization
For the characterization of large AAV aggregates or other large species, field-flow fractionation in combination with MALS (FFF-MALS) is the method of choice.…
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RT-MALS: Process analytical technology (PAT) for AAVs
RT-MALS is a real-time technology for determining empty/full ratio, titer, molar mass and radius. It is valuable in both early- and late stage process…
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